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Research Article | DOI: https://doi.org/10.31579/2637-8914/346
Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
*Corresponding Author: Olujimi John, Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
Citation: Alagbe, Olujimi John, (2025), Evaluation of Bioactive compounds and antimicrobial analysis of the essential oil from the leaves of Sauropus androgynous in Rajasthan India, J. Nutrition and Food Processing, 8(11); DOI:10.31579/2637-8914/346
Copyright: © 2025, Olujimi John. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 02 December 2025 | Accepted: 19 December 2025 | Published: 22 December 2025
Keywords: bioactive; compounds; antimicrobials; free radicals; oxidative stress
Sauropus androgynous essential oil contains unique bioactive compounds with therapeutic potentials and it delivers powerful antioxidant, anti-inflammatory, antidiabetic, cytotoxic, gastro-protective, immune-protective, cardio-protective, antimicrobial, antidiarrheal, anti-helminthic amongst others. GC-MS analysis of Sauropus androgynous essential oil revealed the presence of 33 bioactive compounds were recorded representing 96.27 % composition dominated by Squalene (19.63 %), Caryophyllene (13.92 %), Nonane, 4-methyl (13.82 %) and n-hexadecanoic acid (10.45 %). Other bioactive compounds examined had a concentration less than 3 %. A synergy between the major and minor compounds are working together to protect, heal, restore and improve the overall vitality of the body. The result obtained on the antimicrobial activity of Sauropus androgynous essential oil revealed a high percentage inhibition 77.81 %, 72.00 %, 80.09 %, 73.73 % and 76.26 % against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella flexineri and Salmonella typhi respectively. The results compare well with that of the standard drug (Ciproflaxacin) (65.00 -71.00 %). It was concluded that Sauropus androgynous essential oil contains measurable concentration of compounds that can disrupt the cell membrane of pathogens and will also help to address the increasing cases of antimicrobial resistance.
Sauropus androgynous, an evergreen, multipurpose herb belongs to the Phyllanthaceae plant family which is found in the tropical rain forests of West Africa, although it is also widely distributed in parts of Asia, South America and Australia (Purba and Paengkoum, 2012; Bao et al., 2020). Inside its leasves are bioactive compounds that go far beyond nutrition (Juxian and Xian, 2022; Hernandez and Alagbe, 2025a). The leaves are rich in minerals like calcium, magnesium and iron supporting bone strength and electrolyte balance (Singh et al., 2011; Singh et al., 2021), vitamins (B-complex and C) and protein between 6 – 10 % (Lin et al., 1999; Basker, 2012). For centuries, Traditional healers have used its leaves to soothe infection, skin disorder, pyrexia, bronchitis, diarrhea and relief from acute and chronic infections because its tissues are filled with therapeutic potentials (Samad et al., 2013; John, 2024b).
The plant contains a unique profile of flavonoids and phenolic acids, nature’s compounds known to neutralize the activities of free radicals, reinforces the cardiovascular system by strengthening blood vessel, acts as liver protectors, metabolic regulators, reducing inflammation and restoring enzyme balance (Alagbe, 2024; Singh et al., 2022). They also contains alkaloids, tannins, steroids and saponins which possess several pharmacological functions which includes, cytotoxic, antimicrobial, antitumor, antidiabetic, anti-cancer, antifungal, immune-modulatory, gastro-protective, derma-protective, anti-helminthic, antidiarrheal, anti-allergic, antihistaminic, antipyretic, antiseptic, antispasmodic, antiulcer and antiviral (Naemsuvan, 2013; Zhang et al., 2022). Methanolic extract from Sauropus androgynous leaves and stems show high radical scavenging free activity (Gonzalez et al., 2022; Purba et al., 2022), measurable compounds inhibit the activities of bacteria cells in microbial assays (Caldeira et al., 2004; Chatrou et al., 2012). Its poultices can be used for skins and wounds (Gao et al., 2014; Musa et al., 2020). Aqueous extract suppresses inflammatory cytokines, reducing airway infections and regulating sugar absorption in the body (Adewale et al., 2021; John, 2024a).
Previous studies by Rajeswari et al. (2018) have shown that Sauropus androgynous leaf extract contained 9,12,15-Octadecatrienoic acid at 12.90 %, Ethyl 9,12,15-Octadecatrienoate (15.93 %), Ethyl (9Z,12Z)-9,12-Octadecadienoate (11.28 %), Hexadecanoic acid (13.76 %), l-(+)-Ascorbic acid 2,6-Dihexadecanoate (27.81 %) as the prominent bioactive compounds during GC-MS analysis. Senthamarai and Anusha (2012) also reported that GC-MS profiling in the leaves of Sauropus Androgynus showed that it contained 2(1H) Naphthalenone (41.17 %), Azulene (36.20 %), Pyrene, hexadecahydro (9.07 %), squalene (8.06 %) and 1, 14-Tetradecanediol (2.82 %) as major bioactive compounds. Previous report has shown that that for every newly developed antibiotic, bacterial strains rapidly develop resistance mechanisms against it. Therefore, there is an urgent need for alternative ways to treat bacterial infections and to combat the problem of antimicrobial resistance (Carranza et al. 2015; Hernandez and Alagbe, 2025b). One of the alternatives that can be utilised for combating antimicrobial resistance is traditional herbal remedies from plants. Evaluating the bioactive compounds in Sauropus androgynous oil will further help to unveil their pharmacological and therapeutic properties.
Location of the experiment, plant collection and extraction of Sauropus androgynous oil
This study was carried out at the Microbiology Department Gandhi College of Agriculture, Rajasthan India between October to December 2025. The Institution is situated between 21°35’N 26°09’E East India. Sauropus androgynous fresh leaves were harvested from Rajasthan India and sent to the department of botany, Gandhi College of Agriculture for identification before it was registered under voucher number HU09/2025C/008. The identified leaves were sorted and shade dried for 13 days until a constant weight was achieved. Dried Sauropus androgynous leaves were pulverized using mechanical grinder. Extraction of oil was done by hydrodistillation with a Clevenger-type apparatus according to the procedures outlined by Singh et al. (2021). 250 g of the pulverized Sauropus androgynous was added to 1000 mL of water heated in a glass flask at 60 ℃ for 20 minutes, steam passes via the condenser and when cooled it was collected in a beaker. The oil collected by decantation at the end of the distillation was filtered, dried over column of anhydrous sodium sulfate, and introduced into glass bottles and stored in a refrigerator at 4 °C.
Gas chromatography – mass spectrometry analysis of Sauropus androgynous essential oil
GC/MS analysis of Sauropus androgynous essential oil was done using Claudus 5006 GC-MS Auto Sampler (China) equipped with two silica capillary columns, interfaced with a quadrupole detector (single quadrupole acquisition Method-MS parameters report), source temperature 230°C, Quadrupole temperature 150 °C; the temperature program was 60 °C for 2 min, 60-240 °C at 3 °C/min, then kept at 240 °C during 8 min; injector temperature, 240 °C. The mass spectrometry transfer line temperature, 250 °C; carrier gas, helium at a flow rate of 0.7 ml/min; injection type, split, 20:1; ionization voltage, 70 eV; electron multiplier 1000 eV; scan range 33-400 amu; scan rate, 1.56 scan/s.
Identification of components Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST, 2001) having more than 62,000 patterns. The spectrum of the unknown component was compared with the spectrum of the known components stored in the NIST library.
Antimicrobial assay
The antimicrobial activity of essential oil of Sauropus androgynous essential oil was screened against five standard strains of stock bacteria from the Microbiology department, Gandhi College of Agriculture, Rajasthan. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella flexineri and Salmonella typhi. Microplate Alamar Blue Assay was used to determine susceptibility or resistance of the essential oils to all the selected bacteria strains. Organisms were grown in Mueller Hinton broth and inoculums were adjusted to 0.5 McFarland standard. Essential oil (20 µg/mL) was added in the wells; control wells do not contain essential oil. The volume of 96-well plate was made up to 200 µL. Finally, 5 x106 cells were added in all wells including both control and test. The plate was sealed with parafilm and incubated for 18 - 20 hours. Alamar Blue Dye was dispensed in each well and shaken at about 80 revolution per minute in a shaking incubator for 2 – 3 hours. Plates were covered with foil in shaking incubator. Change in color of Alamar Blue dye from blue to pink indicated the growth in bacterial strains. Ciproflaxacin was used as the reference drug.
Bioactive compounds in Sauropus androgynous essential oil by GC-MS analysis is presented in Table 1. A total of 33 bioactive compounds were recorded representing 96.27 % composition dominated by Squalene (19.63 %), Caryophyllene (13.92 %), Nonane, 4-methyl (13.82 %) and n-hexadecanoic acid (10.45 %). These compounds have been previously associated with anti-inflammatory (Sinha and Munshi, 2011; Namkeleja et al., 2014), antioxidant (Omokore and Alagbe, 2019; Oluwafemi et al., 2021), cytotoxic and immuno-stimulatory properties (Alagbe, 2022; Muritala et al., 2022). According to Shittu and Alagbe (2020), Squalene has the capability to neutralize free radicals’ activities in the body, support blood vessels integrity and protects the heart, lungs and nervous systems. Caryophyllene have been reported to be a proven powerhouse, kills pathogens and inhibits fungal growth (Tiwari et al., 2011; Ojediran et al., 2024). Hexadecanoic acid excels in lowering blood sugar and supports liver detoxification (Sharma, 2012; Jing-Chung et al., 2007; Lima et al., 2010). Nonane, 4-methyl can interfere with mitochondrion energy production in cancer cells, fights infection and calm the nervous system (Choi et al., 2013). All the 33 bioactive compounds have numerous pharmacological properties making them useful in traditional medicine for the treatment of acute and chronic inflammation, cough, fever, gastrointestinal disorders, skin infection, eye problems, sexually transmitted infections, stomach ulcers, pain, snake bite, tooth ache amongst others (Alagbe et al., 2022; Agubosi et al., 2022). A previous study by Senthamarai and Anusha (2012) on the GC-MS analysis of Sauropus androgynous leaf reveals that squalene and phytol contained a concentration of 8.06 % and 0.88 % respectively. Lee et al. (2011) also recorded a lower concentration of 0.12 % and 3.07 % for phytol and hexadecanoic acid. Rajeswari et al. (2018); Basker (2012) reported that Sauropus androgynous leaf extract contained a higher concentration of 13.80 % hexadecanoic acid. Samad et al. (2018) reported 10.40 % and 0.04 % for hexadecanoic acid and 9, 17-Octadecadienal, and these values were almost similar to the outcome in this experiment. However, geographical location, age of plant, specie, storage method, harvesting technique, processing methods have been identified as factors that influence the concentration of bioactive compounds in medicinal plants (Awa et al., 2012). A synergy between the minor and major components in essential oil from Sauropus androgynous in this study forms a rich network of phytochemicals that reduces inflammation by suppressing pro-inflammatory cytokines (Momza et al., 2012; Alagbe et al., 2022), produces bronchodilator effect by opening respiratory airway thereby making breathing easier (Zubairu et al., 2025), improve cardio-vascular health and electrolyte balance thus improving the overall health and vitality of the body (Alagbe, 2025).
The result obtained on the antimicrobial activity of Sauropus androgynous essential oil revealed a high percentage inhibition 77.81 %, 72.00 %, 80.09 %, 73.73 % and 76.26 % against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella flexineri and Salmonella typhi respectively. The results compare well with that of the standard drug (Ciproflaxacin) (65.00 -71.00 %). The presence of squalene, the prominent bioactive compounds in the oil might have been responsible for their antimicrobial activity. Squalene contains measurable compounds that inhibits the activities of some bacteria’s (Ajiboye et al., 2013). Phyto-components like hexadecanoic acid, caryophyllene, cyclopentaneundecanoic acid, 8-octadecenoic acid, humulene, gamma. -Terpinene-3-Carene, 9, 17-Octadecadienal, Undecane, 2,10-dimethyl, 2-methyl-Glutaric acid, 2-Ethylhexyl mercaptoacetate and Linoleic acid ethyl ester have been confirmed to show antibacterial activities (Ajayi et al., 2011). This suggests that dietary supplementation of Sauropus androgynous essential oil can help to reduce the increasing cases of antimicrobial resistance and promote food safety (Ajayi et al., 2011). Paul et al. (2011), essential oil from Sauropus androgynous can defend against infection, disrupts cell membrane of pathogens and inhibited Staphylococcus spp and Salmonella spp in antimicrobial assay. Preveen et al. (2011) reported that Sauropus androgynous oil is potent in controlling bacterial growth in biofilms of Streptococcus spp and Shigella spp.
| Compounds | R.T (min) | Molecular formula | Molecular weight (g/mol) | % Area |
| n-hexadecanoic acid | 16.73 | C16 H32 O2 | 256.424 | 10.45 |
| Dipyrimadole | 18.52 | C24 H40 N8 O4 | 504.626 | 2.31 |
| Octadec -9-enoic acid | 18.90 | C18 H34 O2 | 282.47 | 0.94 |
| N-3-methylButyl acetamide | 18.92 | C7H15NO | 129.20 | 1.46 |
| Benzene,1,4-dichloro | 21.13 | C6H4Cl2 | 147.00 | 0.01 |
| 8-octadecenoic acid | 22.46 | C19 H36 O2 | 296.487 | 0.56 |
| Humulene | 22.78 | C15H24 | 204.35 | 0.18 |
| Undecane, 2,10-dimethyl | 22.98 | C13H28 | 184.36 | 0.46 |
| Oxalic acid | 24.06 | C15H28O4 | 272.38 | 2.77 |
| Nonane, 4-methyl | 25.67 | C10H22 | 142.28 | 13.82 |
| 2-Ethylhexyl mercaptoacetate | 25.75 | C10H20O2S | 247.34 | 1.81 |
| gamma. -Terpinene-3-Carene | 26.31 | C10H16 | 136.23 | 2.28 |
| Hexatriacontane | 28.94 | C36H74 | 507.00 | 2.15 |
| 3-Isothiazolone | 29.07 | C3H3NOS | 101.13 | 1.26 |
| Nerolidol | 29.35 | C15H26O | 222.37 | 0.14 |
| 10-Methylnonadecane | 29.94 | C20H42 | 282.5 | 1.33 |
| Cyclopropane | 30.05 | C3H6 | 42.08 | 2.09 |
| Ethyl palmitate | 31.24 | C18H36O2 | 284.5 | 0.71 |
| 2-methyl-Glutaric acid | 31.88 | C6H10O4 | 146.14 | 0.20 |
| Linoleic acid ethyl ester | 31.90 | C18H32O2 | 308.5 | 0.38 |
| 9,17-Octadecadienal | 33.25 | C18H32O | 264.4 | 0.05 |
| 4-Trifluoroacetoxytetradecane | 33.57 | C16H29F3O2 | 424.4 | 0.57 |
| Hexacosanoic acid | 33.89 | C26H52O2 | 396.7 | 1.10 |
| Beta. -Famesene | 34.21 | C15H24 | 204.35 | 1.59 |
| Cyclopentaneundecanoic acid | 34.57 | C16H30O2 | 254.41 | 1.87 |
| Pentadecane | 37.21 | C15H32 | 212.41 | 0.01 |
| Caryophyllene | 37.67 | C15H24 | 204.351 | 13.92 |
| Trans-Nerolidol | 38.58 | C15H26O | 222.4 | 1.12 |
| Methyl stearate | 38.71 | C19H38O2 | 298.5 | 1.07 |
| Ethyl ricinoleate | 39.12 | C20H38O3 | 326.5 | 0.08 |
| Squalene | 39.65 | C30H50 | 410.7 | 19.63 |
| Hexahydrofarnesyl acetone | 40.19 | C18H36O | 268.5 | 1.24 |
| Phytol | 41.25 | C22H42O2 | 296.5 | 8.71 |
| Total | 96.27 | |||
| Number of compounds | 33.00 |
Table 1: Bioactive compounds in Sauropus androgynous essential oil by GC-MS
| Microorganisms | Ciproflaxacin | % Inhibition |
| Staphylococcus aureus | 71 | 77.81 |
| Escherichia coli | 65 | 72.00 |
| Pseudomonas aeruginosa | 75 | 80.09 |
| Shigella flexineri | 68 | 73.73 |
| Salmonella typhi | 70 | 76.26 |
Table 2: Antimicrobial activity of Sauropus androgynous essential oil
It was concluded that Sauropus androgynous essential oil contains several unique bioactive compounds with therapeutic potentials and has been traditionally utilized for the treatment of cold, fever, inflammation, acute and chronic infections. In vitro studies have also shown that these compounds also have antimicrobial properties and was able to inhibit the activities of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella flexineri and Salmonella typhi. Together they form a weapon against infection, inflammation and degeneration.
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Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.
Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."
I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.
To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.
"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".
I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.
Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.
We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.
My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.
To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina
Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.
Dear Ashley Rosa, Editorial Coordinator of the journal - Psychology and Mental Health Care. " The process of obtaining publication of my article in the Psychology and Mental Health Journal was positive in all areas. The peer review process resulted in a number of valuable comments, the editorial process was collaborative and timely, and the quality of this journal has been quickly noticed, resulting in alternative journals contacting me to publish with them." Warm regards, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. I appreciate the journal (JCCI) editorial office support, the entire team leads were always ready to help, not only on technical front but also on thorough process. Also, I should thank dear reviewers’ attention to detail and creative approach to teach me and bring new insights by their comments. Surely, more discussions and introduction of other hemodynamic devices would provide better prevention and management of shock states. Your efforts and dedication in presenting educational materials in this journal are commendable. Best wishes from, Farahnaz Fallahian.
Dear Maria Emerson, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. I am delighted to have published our manuscript, "Acute Colonic Pseudo-Obstruction (ACPO): A rare but serious complication following caesarean section." I want to thank the editorial team, especially Maria Emerson, for their prompt review of the manuscript, quick responses to queries, and overall support. Yours sincerely Dr. Victor Olagundoye.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. Many thanks for publishing this manuscript after I lost confidence the editors were most helpful, more than other journals Best wishes from, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Agrippa Hilda, Editorial Coordinator, Journal of Neuroscience and Neurological Surgery. The entire process including article submission, review, revision, and publication was extremely easy. The journal editor was prompt and helpful, and the reviewers contributed to the quality of the paper. Thank you so much! Eric Nussbaum, MD
Dr Hala Al Shaikh This is to acknowledge that the peer review process for the article ’ A Novel Gnrh1 Gene Mutation in Four Omani Male Siblings, Presentation and Management ’ sent to the International Journal of Clinical Case Reports and Reviews was quick and smooth. The editorial office was prompt with easy communication.
Dear Erin Aust, Editorial Coordinator, Journal of General Medicine and Clinical Practice. We are pleased to share our experience with the “Journal of General Medicine and Clinical Practice”, following the successful publication of our article. The peer review process was thorough and constructive, helping to improve the clarity and quality of the manuscript. We are especially thankful to Ms. Erin Aust, the Editorial Coordinator, for her prompt communication and continuous support throughout the process. Her professionalism ensured a smooth and efficient publication experience. The journal upholds high editorial standards, and we highly recommend it to fellow researchers seeking a credible platform for their work. Best wishes By, Dr. Rakhi Mishra.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. The peer review process of the journal of Clinical Cardiology and Cardiovascular Interventions was excellent and fast, as was the support of the editorial office and the quality of the journal. Kind regards Walter F. Riesen Prof. Dr. Dr. h.c. Walter F. Riesen.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. Thank you for publishing our article, Exploring Clozapine's Efficacy in Managing Aggression: A Multiple Single-Case Study in Forensic Psychiatry in the international journal of clinical case reports and reviews. We found the peer review process very professional and efficient. The comments were constructive, and the whole process was efficient. On behalf of the co-authors, I would like to thank you for publishing this article. With regards, Dr. Jelle R. Lettinga.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, I would like to express my deep admiration for the exceptional professionalism demonstrated by your journal. I am thoroughly impressed by the speed of the editorial process, the substantive and insightful reviews, and the meticulous preparation of the manuscript for publication. Additionally, I greatly appreciate the courteous and immediate responses from your editorial office to all my inquiries. Best Regards, Dariusz Ziora
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation, Auctores Publishing LLC, We would like to thank the editorial team for the smooth and high-quality communication leading up to the publication of our article in the Journal of Neurodegeneration and Neurorehabilitation. The reviewers have extensive knowledge in the field, and their relevant questions helped to add value to our publication. Kind regards, Dr. Ravi Shrivastava.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, Auctores Publishing LLC, USA Office: +1-(302)-520-2644. I would like to express my sincere appreciation for the efficient and professional handling of my case report by the ‘Journal of Clinical Case Reports and Studies’. The peer review process was not only fast but also highly constructive—the reviewers’ comments were clear, relevant, and greatly helped me improve the quality and clarity of my manuscript. I also received excellent support from the editorial office throughout the process. Communication was smooth and timely, and I felt well guided at every stage, from submission to publication. The overall quality and rigor of the journal are truly commendable. I am pleased to have published my work with Journal of Clinical Case Reports and Studies, and I look forward to future opportunities for collaboration. Sincerely, Aline Tollet, UCLouvain.
Dear Ms. Mayra Duenas, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. “The International Journal of Clinical Case Reports and Reviews represented the “ideal house” to share with the research community a first experience with the use of the Simeox device for speech rehabilitation. High scientific reputation and attractive website communication were first determinants for the selection of this Journal, and the following submission process exceeded expectations: fast but highly professional peer review, great support by the editorial office, elegant graphic layout. Exactly what a dynamic research team - also composed by allied professionals - needs!" From, Chiara Beccaluva, PT - Italy.
Dear Maria Emerson, Editorial Coordinator, we have deeply appreciated the professionalism demonstrated by the International Journal of Clinical Case Reports and Reviews. The reviewers have extensive knowledge of our field and have been very efficient and fast in supporting the process. I am really looking forward to further collaboration. Thanks. Best regards, Dr. Claudio Ligresti
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation. “The peer review process was efficient and constructive, and the editorial office provided excellent communication and support throughout. The journal ensures scientific rigor and high editorial standards, while also offering a smooth and timely publication process. We sincerely appreciate the work of the editorial team in facilitating the dissemination of innovative approaches such as the Bonori Method.” Best regards, Dr. Matteo Bonori.
I recommend without hesitation submitting relevant papers on medical decision making to the International Journal of Clinical Case Reports and Reviews. I am very grateful to the editorial staff. Maria Emerson was a pleasure to communicate with. The time from submission to publication was an extremely short 3 weeks. The editorial staff submitted the paper to three reviewers. Two of the reviewers commented positively on the value of publishing the paper. The editorial staff quickly recognized the third reviewer’s comments as an unjust attempt to reject the paper. I revised the paper as recommended by the first two reviewers.
Dear Maria Emerson, Editorial Coordinator, Journal of Clinical Research and Reports. Thank you for publishing our case report: "Clinical Case of Effective Fetal Stem Cells Treatment in a Patient with Autism Spectrum Disorder" within the "Journal of Clinical Research and Reports" being submitted by the team of EmCell doctors from Kyiv, Ukraine. We much appreciate a professional and transparent peer-review process from Auctores. All research Doctors are so grateful to your Editorial Office and Auctores Publishing support! I amiably wish our article publication maintained a top quality of your International Scientific Journal. My best wishes for a prosperity of the Journal of Clinical Research and Reports. Hope our scientific relationship and cooperation will remain long lasting. Thank you very much indeed. Kind regards, Dr. Andriy Sinelnyk Cell Therapy Center EmCell
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. It was truly a rewarding experience to work with the journal “Clinical Cardiology and Cardiovascular Interventions”. The peer review process was insightful and encouraging, helping us refine our work to a higher standard. The editorial office offered exceptional support with prompt and thoughtful communication. I highly value the journal’s role in promoting scientific advancement and am honored to be part of it. Best regards, Meng-Jou Lee, MD, Department of Anesthesiology, National Taiwan University Hospital.
Dear Editorial Team, Journal-Clinical Cardiology and Cardiovascular Interventions, “Publishing my article with Clinical Cardiology and Cardiovascular Interventions has been a highly positive experience. The peer-review process was rigorous yet supportive, offering valuable feedback that strengthened my work. The editorial team demonstrated exceptional professionalism, prompt communication, and a genuine commitment to maintaining the highest scientific standards. I am very pleased with the publication quality and proud to be associated with such a reputable journal.” Warm regards, Dr. Mahmoud Kamal Moustafa Ahmed
Dear Maria Emerson, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews’, I appreciate the opportunity to publish my article with your journal. The editorial office provided clear communication during the submission and review process, and I found the overall experience professional and constructive. Best regards, Elena Salvatore.
Dear Mayra Duenas, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews Herewith I confirm an optimal peer review process and a great support of the editorial office of the present journal
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. I am really grateful for the peers review; their feedback gave me the opportunity to reflect on the message and impact of my work and to ameliorate the article. The editors did a great job in addition by encouraging me to continue with the process of publishing.
Dear Cecilia Lilly, Editorial Coordinator, Endocrinology and Disorders, Thank you so much for your quick response regarding reviewing and all process till publishing our manuscript entitled: Prevalence of Pre-Diabetes and its Associated Risk Factors Among Nile College Students, Sudan. Best regards, Dr Mamoun Magzoub.
International Journal of Clinical Case Reports and Reviews is a high quality journal that has a clear and concise submission process. The peer review process was comprehensive and constructive. Support from the editorial office was excellent, since the administrative staff were responsive. The journal provides a fast and timely publication timeline.
Dear Maria Emerson, Editorial Coordinator of International Journal of Clinical Case Reports and Reviews, What distinguishes International Journal of Clinical Case Report and Review is not only the scientific rigor of its publications, but the intellectual climate in which research is evaluated. The submission process is refreshingly free of unnecessary formal barriers and bureaucratic rituals that often complicate academic publishing without adding real value. The peer-review system is demanding yet constructive, guided by genuine scientific dialogue rather than hierarchical or authoritarian attitudes. Reviewers act as collaborators in improving the manuscript, not as gatekeepers imposing arbitrary standards. This journal offers a rare balance: high methodological standards combined with a respectful, transparent, and supportive editorial approach. In an era where publishing can feel more burdensome than research itself, this platform restores the original purpose of peer review — to refine ideas, not to obstruct them Prof. Perlat Kapisyzi, FCCP PULMONOLOGIST AND THORACIC IMAGING.
Dear Grace Pierce, International Journal of Clinical Case Reports and Reviews I appreciate the opportunity to review for Auctore Journal, as the overall editorial process was smooth, transparent and professionally managed. This journal maintains high scientific standards and ensures timely communications with authors, which is truly commendable. I would like to express my special thanks to editor Grace Pierce for his constant guidance, promt responses, and supportive coordination throughout the review process. I am also greatful to Eleanor Bailey from the finance department for her clear communication and efficient handling of all administrative matters. Overall, my experience with Auctore Journal has been highly positive and rewarding. Best regards, Sabita sinha
Dear Mayra Duenas, Editorial Coordinator of the journal IJCCR, I write here a little on my experience as an author submitting to the International Journal of Clinical Case Reports and Reviews (IJCCR). This was my first submission to IJCCR and my manuscript was inherently an outsider’s effort. It attempted to broadly identify and then make some sense of life’s under-appreciated mysteries. I initially had responded to a request for possible submissions. I then contacted IJCCR with a tentative topic for a manuscript. They quickly got back with an approval for the submission, but with a particular requirement that it be medically relevant. I then put together a manuscript and submitted it. After the usual back-and-forth over forms and formality, the manuscript was sent off for reviews. Within 2 weeks I got back 4 reviews which were both helpful and also surprising. Surprising in that the topic was somewhat foreign to medical literature. My subsequent updates in response to the reviewer comments went smoothly and in short order I had a series of proofs to evaluate. All in all, the whole publication process seemed outstanding. It was both helpful in terms of the paper’s content and also in terms of its efficient and friendly communications. Thank you all very much. Sincerely, Ted Christopher, Rochester, NY.
